Chemical-analytical investigation of foods and feeds has its origins in the uncovering of adulterations. Many different methods are used today to test authenticity, ranging from classical wet-chemical procedures and modern instrumental techniques to molecular biological methods. Adulterated products are often detected via so-called marker compounds which are characteristic for the detection of a blend or admixture. Examples of this are:
- the detection of horse meat in beef products using PCR analysis
- the detection of the addition of technical glycerine to wine (glycerine is a natural ingredient of wine. The addition of technical glycerine can be determined via the accompanying substances 3-methyl propanol and cyclic diglycerides which are not otherwise found in wine).
One difficulty in the detection of adulterations is the fact that only the substances that are being investigated are usually found, i.e. foods are tested for the presence or absence of various adulterations that are already known. Unknown adulterants can be overlooked. An example of this is the addition of melamine to dairy products to feign a higher protein content, as happened in China in 2009. The protein content in dairy products is usually determined using the Kjeldahl method, with which the total nitrogen content in the sample is determined. It cannot be distinguished using this method whether the nitrogen originates from the milk proteins or from organic, N-containing chemicals such as melamine.
With this in mind, newer approaches also include so-called non-targeted methods which make it fundamentally possible to recognise unknown adulterations, especially unexpected additives. With non-targeted analysis methods, a characteristic fingerprint of a food or feed is taken before checking by a reference library whether it is the claimed food or feed, or not. The possibility of identifying many different deviations from the expected product, e.g. in the form of adulterations, is worthy of special emphasis in the view of the BfRshort forGerman Federal Institute for Risk Assessment, as health and safety relevant adulterations can also be detected in this way. It is hoped that it will also be possible in future to use this method to answer analytically demanding questions, such as the geographic origin of products.